Upcoming presentations at the 2023 Ecological Society of America annual meeting!

Ecological Society of America meeting, Aug 6 – 11, 2023, Portland, Oregon

Scallop microbes and sustainable aquaculture: host-microbe dynamics situated in environmental and social context.

Presentation ID: 1372900

Session Information

Session Title: Microbes as Tools to Solve Ecological Problems for All
Session Type: Inspire Session
Date: Thursday August 10, 2023
Session Time: 3:30 PM – 5:00 PM Pacific Time

Authors: Suzanne L. Ishaq1

Affiliations: 1 University of Maine, School of Food and Agriculture, Orono, ME 04469 USA

Atlantic sea scallop (Placopecten magellanicus) is the second largest fishery in Maine, primarily through wild harvest. Farming is a promising way to meet year-round market demands, create jobs, and reduce ecological impacts of harvest, but relies on wild-caught juveniles as larval survival in hatcheries is low for unknown reasons. My collaborative research group explores the role of larval and tank microbiomes in hatcheries compared to wild scallop veligers. In addition to basic and applied microbiome research, the research team meets with industry partners weekly to discuss results, trends, generate real-world-problem-driven project designs, and collaborate on research, education, and student training.

Bacterial community trends associated with sea scallop, Placopecten magellanicus, larvae in a hatchery system.

Poster ID:  1475974
Poster Title: “Bacterial community trends associated with sea scallop, Placopecten magellanicus, larvae in a hatchery system.”

Session Information

Agriculture
Session Date: Tuesday August 8, 2023 
Session Time: 5:00 PM – 6:30 PM Pacific Time

Authors: Suzanne L. Ishaq1*, Sarah Hosler1, Adwoa Dankwa1, Damian C. Brady2, Erin Grey3, Phoebe Jekielek4, Kyle Pepperman5, Jennifer Perry1, Rachel Lasley-Rasher6, Brian Beal3,7, Timothy J. Bowden1

Affiliations: 1 School of Food & Agriculture, University of Maine, Orono ME 04469. 2 School of Marine Sciences, Darling Marine Center, University of Maine. 3 School of Biology and Ecology, University of Maine, Orono ME 04469. 4 Department of Biological Sciences, University of Southern Maine, Portland ME 04103. 5 Downeast Institute, Beals, ME 04611. Ecology and Environmental Sciences, University of Maine, Orono ME 04473. 7 Division of Environmental & Biological Sciences, University of Maine at Machias, Machias, ME 04654

Atlantic sea scallops, Placopecten magellanicus, are the most economically important marine bivalves along the northeastern coast of North America, and wild-caught adults and juvenile spat are increasingly being cultured in aquaculture facilities and coastal farms. While adults can be induced to spawn successfully in hatcheries, the last two weeks of the larval maturation phase are plagued by large mortality events, making production unfeasible. Research into other scallop- and aquacultured-species point to animal loss from bacterial infections or from altered functionality of host-associated microbiota. There are no previous studies of the bacterial communities from biofilms growing in scallop hatchery tanks, nor even host-microbial studies with this species of sea scallops. We identified bacterial communities in veliger-stage wild larvae, hatchery larvae, and tank biofilms, using the V3-V4 region of the 16S rDNA gene, via Illumina MiSeq sequencing. Hatchery larvae had lower bacterial richness (number of bacteria taxa present) than the wild larvae and tank biofilms, and hatchery larvae had a similar bacterial community (which taxa were present) to both wild larvae and tank biofilms. Bacterial richness was not significantly different between tanks which had been occupied by larvae for 48 hours, and those which had just been drained, scrubbed clean, and refilled with filtered seawater. Static-water-flow compared to continuous-water-flow (flow-through) did not generate different levels of bacterial richness overall, and only an equivocal difference when accounting for time as a smoothing feature in the model (GAM, p = 0.04). Bacterial richness and community similarity between tank samples fluctuated over the trial in repeated patterns of rise and fall, which showed some correlation to lunar cycle  where richness is high when the moon is about 50% and richness is low during new and full moon phases. This may be a proxy for the effects of spring tides and trends in seawater bacteria and phages which are propagated into hatchery tanks. The number of days since the full moon was significantly correlated with bacterial community richness in tanks (GAM, p < 0.01): low during the full moon, peaking ~ 21 days after the full moon, and decreasing again at the next full moon.  These results along with future work, will inform hatcheries on methods that will increase larval survival in these facilities, for example, implementing additional filtering or avoiding seawater collection during spring tides, to reduce certain bacterial taxa of concern or promoting a more diverse microbial community which would compete against pathogens.

Giving the keynote presentation at the Boston University Microbiome Day in July!

I’m honored to be giving the keynote presentation at the Boston University Microbiome Day this July 12! I’ll be sharing my work on microbes and social equity, especially as pertains to health and the environment. You can find event details and registration here. You can also follow the group on Twitter @BuMicrobiome.

TimeEvent
9:00-9:45 AMBreakfast and coffee
9:45-10:00 AMWelcome
Faculty Talks
10:00-10:30 AMDr. Sarah Davies
10:30-11:00 AMDr. Joe Larkin
Keynote Address
11:00AM-12:00 PMDr. Sue Ishaq
12:00PM-1:30 PMLunch and Poster session
Panel Discussion: Translating the Microbiome to Industry
1:30-2:30 PMJennifer Cookson, Andrea Watson and Nili Ostrov:“Translating the Microbiome to Industry”
Student Session Pt 1
2:45-3:05 PMMichael Zulch
3:05-3:35 PMPaul Rousteau
3:35-3:45 PMSession Break
Student Session Pt 2
3:45-4:05 PMCorinne Vietorisz
4:05-4:35 PMMichael Silverstein
5:00 PMSocial Event
 

Upcoming talk at the 9th Southern California Microbiome Symposium in September!

I’m ecstatic to be heading back to southern California this September to present at the 9th annual Southern California Microbiome Symposium. I’ll be sharing my work on microbes and social equity, especially as pertains to food systems and sustainability. Registration is free, and can be found here.

Upcoming presentations at the American Society for Nutrition conference

The Ishaq Lab will be presenting at a few research conferences this summer, with a few more in the works for the fall.

Broccoli sprouts in a tray

American Society for Nutrition meeting, July 22-25, 2023, Boston, Massachusetts

Steamed broccoli sprouts alleviate gut inflammation and retain gut microbiota against DSS-induced dysbiosis.

Poster, abstract P20-022-23, July 23

Authors: Johanna M. Holman*1, Lola Holcomb2, Louisa Colucci3, Dorien Baudewyns4, Joe Balkan5, Grace Chen6, Peter L. Moses7,8, Gary M. Mawe7, Tao Zhang9, Yanyan Li1, Suzanne L. Ishaq1

Affiliations: 1 School of Food and Agriculture, University of Maine, 2 Graduate School of Biomedical Sciences and Engineering, University of Maine, 3 Department of Biology, Husson University, 5 Department of Chemical and Biological Engineering, Tufts University, 6 Department of Internal Medicine, University of Michigan Medical School, 7 Departments of Neurological Sciences and of Medicine, Larner College of Medicine, University of Vermont, 8 Finch Therapeutics, 9 School of Pharmacy and Pharmaceutical Sciences, SUNY Binghamton University.

Objectives: Inflammatory Bowel Diseases (IBD) are devastating conditions of the gastrointestinal tract with limited treatments, and dietary intervention may be effective, affordable, and safe for managing symptoms. Ongoing research has identified inactive compounds in broccoli sprouts, like glucoraphanin, and that mammalian gut microbiota play a role in metabolizing it to the anti-inflammatory sulforaphane. The objectives were to identify biogeographic location of participating microbiota and correlate that to health outcomes. 

Methods: We fed specific pathogen free C57BL/6 mice either a control diet or a 10% steamed broccoli sprout diet, and gave a three-cycle regimen of 2.5% dextran sodium sulfate (DSS) in drinking water over a 40-day experiment to simulate chronic, relapsing ulcerative colitis. We monitored body weight, fecal characteristics, fecal lipocalin, and sequenced bacterial communities from the contents and mucosa in the jejunum, cecum, and colon. 

Results: Mice fed the broccoli sprout diet while receiving DSS performed better than mice fed the control diet while receiving DSS for all disease parameters, including significantly more weight gain (2-way ANOVA, p < 0.05), lower Disease Activity Index scores (2-way ANOVA, p < 0.001), and higher bacterial richness in all gut locations (linear regression model, p < 0.01 for all locations measured). Bacterial communities were assorted by gut location except in the mice receiving the control diet and DSS treatment (Beta-diversity, ANOVA, p < 0.05 for each). Importantly, our results suggested that broccoli sprout feeding completely abrogated the effects of DSS on gut microbiota, as bacterial communities were similar between mice receiving broccoli sprouts with and without DSS. 

Conclusions: Spatially resolved microbial communities provide greater insight when investigating host-microbe interactions. Here, we show that a 10% broccoli sprout diet protects mice from the negative effects of dextran sodium sulfate induced colitis, that colitis erases biogeographical patterns of bacterial communities in the gut, and that the cecum is not likely to be a significant contributor to colonic bacteria of interest in the DSS mouse model of ulcerative colitis. 

Funding Sources: This work was funded by the NIH, USDA, NSF NRT, and UMaine GSBSE.

 

Early life exposure to broccoli sprouts confers stronger protection against enterocolitis development in an immunological mouse model of inflammatory bowel disease.

Poster, abstract P20-021-23, July 23

Authors: Lola Holcomb*1, Johanna Holman2, Molly Hurd3, Brigitte Lavoie3, Louisa Colucci4, Gary M. Mawe3, Peter L. Moses3,5, Emma Perry6, Allesandra Stratigakis7, Tao Zhang7, Grace Chen8, Suzanne L. Ishaq1, Yanyan Li1

1 Graduate School of Biomedical Sciences and Engineering, University of Maine, 2 School of Food and Agriculture, University of Maine, 3 Larner College of Medicine, University of Vermont, 4 Department of Biology, Husson University, 5 Finch Therapeutics, 6 Electron Microscopy Laboratory, University of Maine, 7 School of Pharmacy and Pharmaceutical Sciences, SUNY Binghamton University, 8 Department of Internal Medicine, University of Michigan Medical School.

Objectives: Inflammatory Bowel Diseases (IBD) are chronic conditions characterized by inflammation of the gastrointestinal (GI) tract that burden daily life, result in complications, and disrupt the gut microbiome. Many studies on diet and IBD in mice use an ulcerative colitis model, despite the availability of an immune-modulated Crohn’s Disease model. The objective of this study was to establish IL-10 deficient mice as a model for studying the role of dietary broccoli and broccoli bioactives in reducing inflammation, modifying the immune response, and supporting GI tract microbial systems. 

Methods: Interleukin-10-knockout (IL-10-ko) mice on a C57BL/6 background, beginning at age 4 or 7 weeks, were fed either a control diet or one containing 10% raw broccoli sprouts. Diets began 7 days prior to inoculation with Helicobacter hepaticus, which triggers Crohn’s-like symptoms in these immune-impaired mice, and ran for 2 additional weeks. 

Results: Broccoli sprouts decreased (p < 0.05), fecal lipocalin (LCN2), a biomarker for intestinal inflammation, and fecal blood, diarrhea, and overall Disease Activity Index. Sprouts increased gut microbiota richness, especially in younger mice (p < 0.004), and recruited different communities in the gut (B-diversity, ANOVA, p < 0.001), especially in the colon (B-diversity, ANOVA, p = 0.03). The control group had greater prevalence and abundance of otherwise commensal bacteria which trigger inflammation in the IL-10-ko mice. Helicobacter was within the top-5 most prevalent core genera for the control group, but was not within the top-5 for the broccoli group. Disease parameters and microbiota changes were more significant in younger mice receiving broccoli.

Conclusions : A diet containing 10% raw broccoli sprouts may be protective against negative disease characteristics of Helicobacter-induced enterocolitis in IL-10-ko mice, and younger age is the most significant factor (relative to diet and anatomical location) in driving gut bacterial community richness and similarity. The broccoli diet contributes to prevalence and abundance of bacterial genera that potentially metabolize dietary compounds to anti-inflammatory metabolites in the gut, are bacteriostatic against pathogens, and may ease disease severity.

Funding Sources: This work was funded by the NIH, USDA, NSF NRT, and UMaine GSBSE.

Establishing Growth Curve Assays for Bacterial Glucosinolate Metabolism: A Study Protocol

Poster, abstract P22-030-23, July 23

Marissa Kinney*1, Ryan Wijayanayake1, Johanna Holman1, Timothy Hunt2, Benjamin Hunt 2, Tao Zhang3, Grace Chen4, Yanyan Li1 , Suzanne L. Ishaq1

1School of Food and Agriculture, University of Maine, Orono, Maine, USA 04469; 2 School of Biology and Ecology, University of Maine, Orono, Maine, USA 04469; 3 School of Pharmacy and Pharmaceutical Sciences, SUNY Binghamton University, Johnson City, New York, USA 13790; 4 Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan, USA 48109 

Objective: Inflammatory bowel diseases (IBD) cause dysfunction of the gastrointestinal (GI) tract and can result in hospitalization, suffering and disruption to overall health. Recent work has demonstrated the anti-inflammatory capacity of a broccoli sprout-diet in artificially-induced GI inflammation in pathogen free C57BL/6 mice. Microbiota samples obtained from the GI tract of these mice will be used to study the presence and activity of broccoli glucosinolate hydrolysis to create microbial-sourced bioactives, to further understand the relationship between broccoli-diets and inflammation reduction. It is imperative to validate or replicate qPCR protocols which have been established for glucosinolate metabolism in Bacteroides thetaiotaomicron (B. theta), in other bacterial species. Additionally, this project will focus on developing new growth curve assays for glucosinolate metabolism, as these methods are lacking in published literature. 

Methods: Pathogen free C57BL/6 mice were given dextran sodium sulfate (DSS) into their drinking water to create a disease profile similar in development and morphology to human ulcerative colitis (UC), a type of IBD. DSS and a steamed broccoli sprout diet were administered. Samples were taken from the digesta, jejunum, cecum, and colon of mice fed broccoli diets. About 806 bacterial isolates will be grown up/cultured anaerobically on minimal/selective media containing glucosinolate-related compounds (glucoraphanin, sinigrin) to determine hydrolysis activity via spectroscopy to measure optical density of growth in competent isolates. Successful isolates will be further analyzed with LC/MS to confirm production of bioactive products, and with qPCR using the B. theta positive control genome to help identify gene targets (α-1,6-mannanase, glycosyl hydrolase, nicotinamide-dependent oxidoreductase, and transcriptional regulator protein) for glucosinolate conversion in isolates. 

Results  N/A

Conclusions: In the initial study from which these samples are sourced, mice fed a broccoli diet had less inflammation than those fed a control diet and DSS, and had higher bacterial diversity in their gut. We expect that bacteria isolated from the GI of broccoli-fed mice will contain more glucosinolate-metabolizing genes.

Funding: NIH (Li and Ishaq) and USDA (Li). 

Broadening Perspectives by Situating Nutrition Education in Broader Social Contexts: A Study Protocol

Poster, abstract number 1490287, July 23

Authors: Ashley Toney*1, Patricia Wolf*2, Sue Ishaq*3

Affiliations: 1Dept. of Cardiovascular and Metabolic Sciences, Cleveland Clinic Lerner Research Institute, 2 Department of Nutrition Science, Purdue University, 3 School of Food and Agriculture, University of Maine

Objectives: The Microbes and Social Equity (MSE) Working Group connects microbiology with social equity research, education, policy, and practice to understand the interplay of microorganisms, individuals, societies, and ecosystems. Given the complexity and nuance of evidence-based nutrition delivery, MSE sought to provide a conceptual structure. The goals are 1) convene diverse researchers, educators, learners, and practitioners, 2) publish and present evidence-based information within socio-cultural contexts, and 3) teach audiences to define ways for integrating equity into their work.

Methods: MSE hosted a 14-week speaker series in 2021, 2022, and 2023, with researchers from various disciplines (e.g., nutrition, gut microbes, food security). The series lead into a 5-day symposium of speakers and guided discussions to generate co-written documents identifying research needs and resources. We use targeted and non-targeted event promotion to attract audiences, and reach/impact is evaluated through registration, attendance, social media views, or attendee feedback. The series provides learning sessions that build concepts over time, guiding attendees past the conceptual roadblock of being new to interdisciplinary research and grappling with grand challenges. This extended period to learn stimulates participation in activity-based collaboration during symposia, even from new members or students. 2023 symposium attendees will be surveyed on their impressions on the event, how it impacted their perspective on experimental design, whether attendees started with the series and followed up with the symposium, and whether having the preface of the series aided in being able to create actionable outputs in a guided co-working session.

Results: n/a

Conclusions: Benefits include nuanced knowledge/perspective sharing, establishing and nurturing interdisciplinary collaboration, and sparking conversations on critical topics in policy, sustainability, host and microbial metabolism, etc. In the last 3 years, MSE published 4 papers together, with ~ 50 independent papers from members. Event recordings are used as curriculum in nutrition, microbiology, and pre-med biology.

Funding Sources: NIH/NIDDK, Allen Foundation (Ishaq); National Dairy Council (Toney)

MSE speaker series starts next week, features talks on diet and gut microbes, early life and breastfeeding, soil health, equitable research, how microbes are part of us, and more!

The Microbes and Social Equity Speaker Series starts next week! Join us for talks on microbiomes and health, equity in research, and more!

Spring 2023; January 18 – May, Wednesdays from 11:00 AM – 12:00 PM EST.

Presented over Zoom. Registration is free!

You can register for any or all of the events from the same link here.


Gut microbiome, nutrition, and food security

Theme organized by Sue Ishaq

“Broccoli Sprout Bioactives and Gut Microbiota: A Dietary Approach for Prevention and Management of Inflammatory Bowel Disease”

Dr. Yanyan Li, PhD

January 18, 2023; Wednesday,11:00 AM – 12:00 PM EST. Register for the Zoom link here.

Headshot of Dr. Yanyan Li

Dr. Li is an Assistant Professor at the University of Maine. She received her PhD degree in Nutrition and Food Science from Ohio State University. She has been dedicating herself to studying the mechanisms of diet-derived bioactives in protecting against disease process and harnessing the gained knowledge to develop dietary approaches for disease prevention and management for more than a decade. Since 2016, she has been focusing on the interactions between dietary components, in particular glucosinolates from cruciferous vegetables such as broccoli and broccoli sprouts, and gut microbiota, aiming to develop a combined approach for inflammatory bowel disease. Her current research projects are funded by NIH/NIDDK, USDA/NIFA AFRI Foundational Program, and nutrition research programs of private foundations.

Added by Sue: For the past few years, Yanyan and her colleagues have also included the Ishaq Lab, and has led to a rewarding and productive collaboration which has resulted in several recent and forthcoming publications, funding awards, and students trained.

“Exploring Health Determinants, Gut Microbiome, and Health Outcomes in Immigrants”

Dr. Dany Fanfan, Ph.D., MSN, RN

January 25, 2023; Wednesday,11:00 AM – 12:00 PM EST. Register for the Zoom link here.

Headshot of Dr. Dany Fanfan

Dr. Dany Fanfan is an Assistant Professor at the University of Florida (UF) College of
Nursing. Before becoming a faculty, she completed a Bachelor’s degree in Nursing at
Florida International University, Master’s and Doctoral degrees in Nursing at the
University of South Florida, and a post-doctoral fellowship at UF focused on mental
health research with and for underrepresented populations (e.g., Latino/Haitian
immigrant farmworkers, rural Latino/LGBTQ+ adolescents) using a community-based
participatory research approach and social network analysis. She teaches and engages in
multidisciplinary mixed-methods research dedicated to advancing the science and
practice of reducing mental health disparities among minoritized immigrants by
exploring the underlying biobehavioral, cultural, and psychosocial mechanisms of
distress symptoms. With support from an NIH K23 career development award, she is
now incorporating microbial metagenomics and bioinformatics methods in her research
by examining the associations between post-migration social determinants of health,
gut microbiome, and psychological distress among recent Haitian immigrants. The long-
term goal of her interdisciplinary translational program of research is to identify and
address the conditions that create and sustain health disparities in minoritized
populations as well as develop and test culturally responsive interventions that target
social, behavioral, and biological determinants of health to improve long-term health
outcomes, reduce behavioral and mental health disparities, and increase health equity.

“Personalized nutrition and the human gut microbiome”

Dr. Sean Gibbons, PhD

February 1, 2023; Wednesday,11:00 AM – 12:00 PM EST. Register for the Zoom link here.

Headshot of Dr. Sean Gibbons.

Dr. Sean Gibbons is an Associate Professor at the Institute for Systems Biology, a non-profit research consortium. His lab develops computational and experimental tools for exploring and manipulating host-microbe systems.

Added by Sue: The work from Sean’s group and collaborators has been reshaping the way that host microbial researchers approach their work, by revealing trends through large metanalyses and novel perspectives on using data. Their most recent work has evaluated host-microbial interactions, metabolites, and health.

For the last three years, Sean’s lab has hosted the ISB Virtual Microbiome Series, which is freely available and attracts several thousand participants. The series includes a two day workshop that teaches data analysis skills, and a day-long symposium featuring discussions of current discoveries and conceptualizes the future of microbiome research.

Finally, Sean and his research group have been making science a more welcoming and inclusive place.

Panel discussion on Gut microbiome, nutrition, and food security

February 8, 2023; Wednesday,11:00 AM – 12:00 PM EST. Register for the Zoom link here.

This week, we’ll be bringing all of our Theme 1 speakers back to engage in a panel discussion together on the gut microbiome. Panel will be hosted by Sue Ishaq.

Please note, this session will only be featured live in real-time and will not be recorded.



Prenatal to early-life microbes and health

Theme organized by Emily Wissel.

Speaker confirmed but time TBD:

Dr. Eldin Jašarević, PhD. Magee-Womens Research Institute, University of Pittsburgh School of Medicine

Speaker 4 TBD

February 15, 2023; Wednesday,11:00 AM – 12:00 PM EST. Register for the Zoom link here.

“Intersecting breastmilk and microbiome science with the complexity of working with humans in a clinical context”

Dr. Merilee Brockway, PhD RN IBCLC, University of Calgary

February 22, 2023; Wednesday,11:00 AM – 12:00 PM EST. Register for the Zoom link here.

Dr. Merilee Brockway is a PhD prepared nurse and International Board-Certified Lactation Consultant (IBCLC) with expertise in maternal-child health, infant feeding, and patient engagement. She completed my PhD in nursing at the University of Calgary, examining maternal breastfeeding self-efficacy and infant feeding outcomes in moderate and late preterm infants. She also completed a three year post-doctoral fellowship at the University of Manitoba in Dr. Meghan Azad’s THRIVE Discovery Lab, exploring clinical applications of donor human milk for preterm infants. As an Assistant Professor at the University of Calgary, her program of research examines the use of human milk as a clinical intervention to mitigate early life perturbations to the infant microbiome.  

Speaker 6 TBD

March 1, 2023; Wednesday,11:00 AM – 12:00 PM EST. Register for the Zoom link here.

Panel discussion on Prenatal to early-life microbes and health

March 8, 2023; Wednesday,11:00 AM – 12:00 PM EST. Register for the Zoom link here.

This week, we’ll be bringing all of our Theme 2 speakers back to engage in a panel discussion together on the microbiome in early life. Panel will be hosted by Emily Wissel.

Please note, this session will only be featured live in real-time and will not be recorded.



The environment, microbes, and us

Anthropology Theme organized by Katherine Daiy and Kieran O’Doherty, and Environmental Theme organized by Mallory Choudoir, Mustafa Saifuddin, and Hannah Holland-Moritz.

Speakers confirmed by time TBD:

  • Dr. Stephanie Schnorr, University of Vienna, “The human-valued interest in microbiome science is the distillation of human-environmental interactions”

“Microbiome Research with the Yanomami”

David Good, University of Guelph

March 22, 2023; Wednesday,11:00 AM – 12:00 PM EST. Register for the Zoom link here.

David Good

David Good is a PhD student in microbiology at the University of Guelph, Ontario. His general research goal is characterizing the structural and functional microbial diversity of his Yanomami family, the Irokae-teri, located in the Amazon rainforest of Venezuela. They are of great interest in the microbiome field since the Irokae-teri live fully immersed in the rainforest environment and subsist by an active lifestyle of hunting-gathering and small-scale gardening. Furthermore, their relative isolation deep in the Amazon limits their exposure to microbiome stressors such as antibiotics, highly refined and processed foods, industrial toxins and pollutants, food preservatives, etc. David will discuss this unique and rare opportunity to advance our understanding of the human microbiome of a community largely unperturbed by westernization, while building global awareness on the importance of protecting these few remaining isolated indigenous societies. However, such research brings numerous challenges surrounding bioethics. David hopes to build dialogue around going beyond simple compliance in microbiome research, and how the Yanomami have the right to self-determination and harness their bioeconomic potential to protect their home.

Photo sourced from: https://www.jointhegoodproject.org/team

“Religion, Race and the Microbe: Theological Analysis of Public Health Resistance in the Pandemicine”

Dr. Aminah Al-Attas Bradford, PhD.

March 29, 2023; Wednesday,11:00 AM – 12:00 PM EST. Register for the Zoom link here.

Dr. Bradford is a research scholar in NC State’s Public Science Lab for Ecology, Evolution and Biodiversity of Humans and Food where she draws together interdisciplinary engagement of microbes, exploring fermentation, probiotic health and pathogens. Working at the intersections of religion, microbiology, ecology and race, Dr. Bradford’s research investigates the historical entanglement of disease theories, public health strategy, Christian thought, and coloniality to cultivate ecological wisdom, scientific engagement and the pursuit of environmental justice in religious contexts. She asks questions like, how have the historical entanglement of epidemiology, coloniality and Christian teaching contributed to the disease of both body and planet, the disproportionate effects of which are born by black and brown communities? How has demonizing the microbe paved the way for oppression of those deemed sub-human? And how might microbiome science reform Christian thought that often disrupts engagement of science and is complicit in exploitative and exclusionary ways of being?

Speaker 9 TBD

April 5, 2023; Wednesday,11:00 AM – 12:00 PM EST. Register for the Zoom link here.

“Anthropology, Microbiomes, and Antimicrobial Resistance”

Dr. Cecil Lewis, PhD.

April 12, 2023; Wednesday,11:00 AM – 12:00 PM EST. Register for the Zoom link here.

Dr. Cecil Lewis is a Professor at the University of Oklahoma.

Image sourced from: https://cecilmlewis.com/

Dr. Anna Krzywoszynska, PhD.

April 19, 2023; Wednesday,11:00 AM – 12:00 PM EST. Register for the Zoom link here.

Dr. Anna Krzywoszynska is starting a position as an Associate Professor of Transdisciplinary Human-Environment Relations, Faculty of Humanities, University of Oulu, Finland.

Speaker 12 TBD

April 26, 2023; Wednesday,11:00 AM – 12:00 PM EST. Register for the Zoom link here.

Panel Discussion on the environment, microbes, and us

May 3, 2023; Wednesday,11:00 AM – 1:00 PM EST. Register for the Zoom link here.

This week, we’ll be bringing all of our Theme 3 speakers back to engage in a panel discussion together on the importance of environmental microbiomes and our place in ecosystems, and then will continue talking about soil health. Panel will be hosted by Katherine Daiy, Kieran O’Doherty, Mallory Choudoir, Mustafa Saifuddin, and Hannah Holland-Moritz.

Please note, this session will only be featured live in real-time and will not be recorded.


Logo designed by Alex Guillen

Happening today ‘Session 5: MSE Education Practices and Curriculum Design’ at the July 2022 MSE virtual symposium!

Today is the fifth and final day of the July 2022 MSE virtual symposium, which is focused on “MSE Education Practices and Curriculum Design”. Don’t worry, you still have time to register!

This session will feature three talks featuring educators who have brought sociology into their microbiome courses, and vice versa, and who have experience creating out-of-the-box curricula to engage students in learning while helping them to see themselves as scientists. Our hope is that attendees for this session learn from different perspectives how to creatively present microbiology courses which situate learning about the microbiome with learning about social and environmental systems.

Session 5: “MSE Education Practices and Curriculum Design”

Friday, July 22nd, 12:30 ~ 16:00 EST. post updated, event has passed, watch the recorded talks.

Session leaders:

Erin Eggleston, PhD, Assistant Professor of Biology, Middlebury College

Monica Trujillo

Monica Trujillo, Ph.D., Associate Professor, of Biology Queensborough Community College, The City University of New York

Carla Bonilla, Ph.D., Assistant Professor, University of San Diego

Scope: Curriculum which blends disciplines is highly engaging, and can be used to teach complex concepts, and can help students combine their existing cultural and social identities with their growing researcher identity. However, creating an interdisciplinary curriculum can be challenging. This session frames educational conversations in MSE, and gives perspectives on creating courses that blend microbiome and social sciences for different levels of education.

Learning Objectives of Session: Attendees will 1) identify successes and barriers to entry for MSE curriculum at different education levels (K-12, UG, grad, general public), 2) Share ways in which we incorporate MSE in our curricula (i.e. assignments, class period, multi-day module, full course, etc.); 3) develop ideas for further curriculum design for their own courses.

Format of talks: Three 30-min lecture-style talks from education practitioners who have successfully built courses around MSE topics, including an outline of learning goals, approach to course, lessons learned/challenges, and more.

Format of breakout rooms: Each room creates a lesson plan outline, and each room has a designated topic area (e.g. human microbiome equity) to help audience members group by teaching discipline.

Session Speakers

Sarah Miller

Sarah Miller, M.S., Executive Director of Tiny Earth at University of Wisconsin-Madison

“Tiny Earth: Leveraging an instructor community to create antiracist curriculum in a research course”

Dr. Ally Hunter
Dr. Melissa Zwick

Dr. Ally Hunter, PhD., Lecturer, iCONS Program & Postdoctoral Fellow, Center for Youth Engagement, University of Massachusetts, Amherst. Part of NSF Project RAISE (Reclaiming Access to Inquiry Science Education for Incarcerated Learners), and NSF Project INSITE (INtegrating STEM Into Transition Education for Incarcerated Youth).

Dr. Melissa Zwick, PhD., Associate Professor of Biology, Stockton University

“Science through storytelling:  Using case study pedagogy as inclusive practice in undergraduate microbiology.” 

Dr. Davida Smyth

Dr. Davida Smyth, Ph.D., Associate Professor of Molecular Microbiology at Texas A&M University in San Antonio

“Using wicked problems to CURE your teaching”

12:30 – 14:15 Introduction and Speakers

14:15 – 14:30 Break

14:30 – 16:00 Breakout room discussions based on skills development, in smaller groups

  • Undergraduate microbiology courses resources/MSE integration
  • Pedagogy as scholarship/publishing mechanisms/resources
  • Assessing case study style teaching

Prior to this session, you may want to watch these recorded talks:



Happening today, ‘Session 4: Community engagement and collaboration’ at the July 2022 MSE virtual symposium!

Today is the fourth day of the July 2022 MSE virtual symposium, which is focused on “Community engagement and collaboration”. Don’t worry, you still have time to register and join the conversation!

This session will feature four talks featuring researchers who have experience bringing communities and members of the public into research teams as contributing members, rather than just study subjects. Not only does this improve the relationship between research and the public, but it creates better-informed research studies and wider spread of positive impacts. Our hope is that attendees for this session learn from different perspectives how to engage with communities early on to spark conversations and collaboration with their research.

Session 4: “Community engagement and collaboration”

Thursday, July 21st, 12:30 ~ 16:00 EST. post updated: This session has passed, watch the recorded talks.

Session leaders:

Portrait of Mustafa Saifuddin, Ph.D.,

Mustafa Saifuddin, Ph.D., Staff Scientist, Sustainable Food and Farming Program at Earthjustice

Ashley M. Toney, Ph.D., Postdoctoral Research Fellow, UTHealth School of Public Health, El Paso. Translational/Clinical Nutrition Researcher focused on Latine Health Disparities.

Scope: Due to the interconnectedness of microbial processes and social justice, many types of microbial research could benefit from closer collaborations with communities impacted directly by the public health, environmental and climate justice implications of microbiomes. Some styles of microbiome research would yield more positive outcomes if the collaboration was built around mutual long-term goals, instead of specific projects, and if it was initiated during project conceptualization instead of after the project has been designed. This session will explore different styles of interdisciplinary collaborations centered on community needs, such as community advisory boards, community partnerships, community-led research design, and how to implement this into microbiome research.

Learning Objective of Session: Attendees will learn 1) approaches to community-centered collaborations, 2) how to leverage community professionals (e.g. health workers) in a ‘train the trainer model’, 3) how to start ethical conversations around environmental samples & broader experimental design, and 4) how to emphasize collaborations – including public health, government, policy makers, etc. as a collaborator and how to ask for their help/mindful collaborations.

Format of talks: Four 30-min lecture-style talks from researchers who have successfully built research collaborations with communities.

Format of breakout rooms: Each room creates a plan for engagement, and each room has a designated topic area (e.g. environmental restoration) to help audience members group by research discipline.

Session Speakers:

Dr. Pajau (PJ) Vangay, PhD. Science Community Manager, National Microbiome Data Collaborative, Lawrence Berkeley National Laboratory.

“Advancing microbiome science, in partnership with communities”

Dr. Rosie Alegado

Dr. Rosie Alegado, PhD., Associate Professor, Oceanography; Director, Sea Grant Ulana ʻIke Center of Excellence; Director, School of Ocean and and Earth Science and Technology Maile Mentoring Bridge Program at the University of Hawaiʻi, Mānoa

“Community-embedded microbiology in Indigenous spaces”

Dr. Arbor Quist

Dr. Arbor Quist, PhD., Postdoctoral Fellow in Environmental Justice & Community-Driven Epidemiology in the Department of Population and Public Health Sciences at the University of Southern California.

“Partnering with Communities in Environmental Disaster Research”

Professional headshot of Dr. Aidee Guzman

Dr. Aidee Guzman, PhD., NSF and UC Chancellor’s Postdoctoral Fellow in the Department of Ecology and Evolutionary Biology at UC Irvine.

“Building agricultural resilience from the ground up.”

12:30 – 14:15 Introduction and Speakers

14:15 – 14:30 Break

14:30 – 15:00 Fourth speaker

15:00 -16:00 Breakout room discussions based on skills development, in smaller groups

  • Community-based participatory research (CBPR) & Scientific Community Engagement
  • Best engagement practices (and things to avoid)
  • Community driven epidemiology
  • Agricultural community engagement
  • Finding a community to engage

Prior to this session, you may want to watch these recorded talks:

Happening today, ‘Session 3: Transforming your research for policy engagement’ at the July 2022 MSE virtual symposium!

Today is the third day of the July 2022 MSE virtual symposium, but don’t worry, you still have time to register for the session. Today’s session is focused on “Transforming your research for policy engagement”. This session will feature three talks featuring researchers who have experience bringing research to the public and to legislative bodies. So often, the positive outcomes of research are limited because it can be difficult to get the word out to people who can put our results into practice. Our hope is that attendees for this session learn from different perspectives how to write their research to inform the general public, professionals in healthcare, or policy makers.


Session 3: “Transforming your research for policy engagement”

Wednesday, July 20th, 12:30 ~ 16:00 EST. Post updated: watch the recorded talks here.

Section leaders:

Mallory Choudoir, Ph.D. Soil microbial ecologist. Postdoctoral Researcher at University of Massachusetts Amherst. Assistant Professor and Extension Specialist at North Carolina State University September 2022. 

Mustafa Saifuddin, Ph.D., Staff Scientist, Sustainable Food and Farming Program at Earthjustice

Mustafa Saifuddin, Ph.D., Staff Scientist, Sustainable Food and Farming Program at Earthjustice

Amali Stephens, PhD Student, Interdepartmental Microbiology, Iowa State University

Scope: Microbiomes drive processes in all environments and are intimately intertwined with all aspects of our lives. Despite the central role of microbes in shaping systems, microbial researchers are often detached from shaping policies related to conservation, public health, land use, environmental justice, climate and other areas of intersection. Policy engagement is not typically included in the academic training of microbiome researchers, and there is a need for greater coordination between policy needs and microbial research. This session will explore integrated, collaborative approaches to research and policy making.

Learning Objectives of Session: Attendees will discuss 1) how to develop research in collaboration with policy needs, 2) policy levels and types (government, private), 3) how to identify stakeholders, and 4) how to communicate your research to policymakers.

Format of talks:  Three 30-min lecture-style talks will describe interdisciplinary research outcomes which transcend typical academic endpoints and engage in shaping policy.

Format of breakout rooms: Each room will create a policy brief outline or ideas list around a particular topic area (e.g. environmental restoration) to help audience members group by discipline.

Session Speakers: In development, details provided soon!

Dr. Caitlyn Hall

Dr. Caitlyn Hall, PhD., Assistant Professor of Practice, University of Arizona

“The Elephant in the Lab: How can scientists engage in policy and advocacy?”

Dr. Kathleen Treseder

Dr. Kathleen Treseder, PhD., Howard A. Schneiderman Endowed Chair and Professor of Biology at the University of California Irvine; Climate Activist; Irvine City Council Candidate

“My experience advocating for environmental policy with local policy makers: What worked, what didn’t.”

Dr. Sonja Birthisel

Dr. Sonja Birthisel, Ph.D., Faculty Associate, University of Maine School of Forest Resources and Ecology & Environmental Sciences Program; Director, The Wilson Center at the University of Maine; Councilor, Orono Maine Town Council

“Public Policy Engagement & Personal Sustainability: What’s Your “Sparkle Zone”?”

12:30 – 14:15 Introduction and Speakers

14:15 – 14:30 Break

14:30 – 16:00 Breakout room discussions based on skills development, in smaller groups

  • How to talk to your politicians about science
  • How scientists get involved with policy
  • Curriculum for science policy
  • Environmental microbial policy issues
  • Microbial conservation
  • Soil carbon & climate justice issues
  • Agricultural antibiotic use
  • Microbial exposures (residential, worker exposure)

Prior to this session, you may want to watch these recorded talks:

You might also be interested in:

Dr. Monica Trujillo, Associate Professor, MSE member, and Symposium session leader, will be at #ASMhillday on July 26th to educate policymakers on microbiology, climate change, and policy.

Happening today: ‘Session 2: Blending biological, social, and humanities writing’ at the July 2022 MSE virtual symposium!

Today the second day of the July 2022 MSE virtual symposium, which is focused on “Blending biological, social, and humanities writing”. Don’t worry, you still have time to register and join the session.

This session will feature one talk and one panel discussion, featuring researchers who have published, reviewed, and edited interdisciplinary writing and appreciate the difficulty that many microbiome researchers face: getting their work published when it does not fit a typical experimental layout. Our hope is that attendees for this session learn from different perspectives how to write across disciplines, find the right journal and pitch the relevancy of their manuscript to the journal’s scope, how to find reviewers with disparate professional backgrounds (for example microbiology and legal policy), and more.


Session 2: “Blending biological, social, and humanities writing”

Tuesday, July 19th, 12:30 ~ 16:00 EST. post updated, this event has passed, watch the recorded talks here.

Session leaders:

Ashley Toney, PhD

Ashley M. Toney, Ph.D., Postdoctoral Research Fellow, UTHealth School of Public Health, El Paso. Translational/Clinical Nutrition Researcher focused on Latine Health Disparities.

Dr. Kieran O'Doherty.

Kieran C. O’Doherty, PhD., Professor of Psychology, University of Guelph, and Director of the Discourse, Science, Publics research Group

Emily Wissel, Ph.D. candidate, Emory University. MSE Director of Resource Dissemination

Scope: Interdisciplinary experimental designs have been called for in research, but finding a publication venue can be tricky when manuscripts or presentations are deemed not discipline-specific, or are labeled opinion instead of research. This session will explore common gatekeeping problems of interdisciplinary research, cross-disciplinary writing categorization discussions (i.e. theoretical framing, etc.), and writing strategies and publication venues to make the most of your work.

Learning Objectives of Session: Attendees will become familiar with different expectations within research design/publishing across fields, and learn about tangible suggestions from research publishers. Audience members should walk away with more confidence in interdisciplinary publishing.

Format of talks: This will feature a 30-min plenary topic to introduce the concept that theory in psychology/philosophy is regarded as opinion in the natural sciences, followed by 1 hour of a panel of research journal editors to discuss flexible publication guidelines.

Format of breakout rooms: Each room creates a document, and each room has a designated topic area (e.g. environmental restoration) to help audience members group by discipline

Session Speaker:

Dr. Mark Risjord, PhD. Professor of Philosophy, Emory University

“Crossing boundaries, building bridges: some reflections on interdisciplinary writing.”

After which, the Speaker will be joined by additional Panelists to discuss interdisciplinary research, challenges, and opportunities.

Dr. Susan L. Prescott, MD, PhD, FRACP. President, inVIVO Planetary Health @ the Nova Institute for Health, Baltimore, USA; Director, ORIGINS PROJECT Telethon Kids Institute; Professor of Paediatrics, UWA Medical School; Paediatric Immunologist, Perth Children’s Hospital; Editor in ChiefChallenges journal.

Dr. James Stegen

Dr. James Stegen, PhD., Physical & Computational Sciences Directorate, Pacific Northwest National Laboratory

Dr. Michela Gambino, professional headshot

Dr. Michela Gambino, PhD. Assistant Professor at the Department of Veterinary and Animal Sciences, University of Copenhagen; mSystems editor

12:30 – 14:15 Introduction, Speaker, and Panel discussion

14:15 – 14:30 Break

14:30 – 16:00 Breakout room discussions based on skills development, in smaller groups

  • Pitching your paper to the right journal 
  • Finding and directing reviewers
  • “Ask a philosopher!”
  • TBD

Prior to this session, you may want to watch these recorded talks:


Happening today: ‘Session 1: Context-aware experimental designs’ at the July 2022 MSE virtual symposium!

Today is first day of the July 2022 MSE virtual symposium, which is focused on “Context-aware experimental designs”! Don’t worry, you still have time to register for the meeting.

The three talks, featuring a total of 5 researchers, will present perspectives on the human microbiome and studying it within broader contexts to better understand our interactions with microbes. Our hope is that attendees for this session learn from different perspectives how to more creatively design or analyze their research to account for the effects that social policy and local environment can have on microbial exposures.

Session 1: “Context-aware experimental designs”

Monday, July 18th, 12:30 ~ 16:00 EST. post updated: This session has passed, watch the recorded talks.

It’s free, and held over Zoom.

Session leaders:

Dr. Ariangela Kozik, Ph.D., Postdoctoral Research Fellow, University of Michigan, and the Co-founder and Vice President of the Black Microbiologists Association

Sue Ishaq

Dr. Sue Ishaq, Ph.D., Assistant Professor at University of Maine and Founder of the Microbes and Social Equity working group.

Scope: Microbiome research often uses broad categorical factors as proxy factors for complex social or environmental contexts, but these can ignore or obscure underlying trends. This session will unpack proxy terms like race, Western diet, dysbiosis, rural/urban, and more, to differentiate what variables we actually want to measure and how to accomplish this in data collection and analysis. This session will also discuss how to communicate microbiome results in relation to broader contexts of lived experiences, rather than attributing results to broad proxy categories.

Learning Objectives of Session: Attendees will learn 1) the process of identifying more precise and appropriate measurement variables when engaging in human-adjacent microbiome research, instead of using proxy factors, 2) how to include more resolution to factorial data during collection, and 3) examples of how to process complex social data during microbiome data analysis.

Format of talks: Three 30-min lecture-style talks will disambiguate proxy categorizations into more precise variables that consider social contexts, approach to course, lessons learned/challenges.

Format of breakout rooms: Each room creates a concept map which disambiguates a proxy category into specific variables, and discusses how to frame surrey questions or leverage existing data to obtain this information. Each room has a designated topic area (e.g. environmental restoration) to help audience members group by discipline or type of information they are looking for.

Session Speakers:

Dr. Elizabeth Roberts.

Dr. Elizabeth F.S. Roberts, PhD., Professor of Anthropology, University of Michigan

Making better numbers through bioethnography

Dr. Katherine Maki, PhD., Assistant Clinical Investigator, Translational Biobehavioral and Health Disparities Branch, National Institutes of Health Clinical Center

Dr. Nicole M. Farmer, M.D., Principal Investigator, Translational Biobehavioral and Health Disparities Branch, NIH Clinical Center

Dr. Kelly K. Jones, Ph.D., RN, Research Fellow, Neighborhoods and Health Lab, Division of Intramural Research, National Institutes of Health

“Proposal of Neighborhood Socioeconomic Status Based Analysis of Human Microbiome Project”

Dr. Osama Tanous, M.D., Palestinian pediatrician based in Haifa and a board member of Physicians for Human Rights – Israel; Visiting Scientist, FXB Center for Health and Human Rights, Harvard University; Hubert H. Humphrey Fellow of Public Health and Health Policies, Emory University. His recent publication can be found here.

“From bedside to the journal – understanding bacteria in a settler colonial setting”

12:30 – 14:15 Introduction and Speakers

14:15 – 14:30 Break

14:30 – 16:00 Breakout room discussions based on skills development, in smaller groups

  1. Deconstructing race as a biological variable
  2. Common pitfalls/challenges to experimental design 
  3. Matching clinical work to social contexts.
  4. Bioethnography to generate hypotheses
  5. Planning for variables in microbiome and social research
  6. Combining microbiome and social data analysis
  7. TBD

Prior to this session, you may want to watch these recorded talks:


Featured image from Robinson et al. 2022.