5-year anniversary of my PhD graduation!

Seems like a lifetime ago that I walked the stage and was hooded during the graduation for my doctoral degree, just 5 years ago. In 2020, most Universities have cancelled their in-person graduations due to pandemic concerns, with faint hopes that they might be able to host the opportunity for 2020 graduates “to walk” at a future ceremony. It’s a good day to reflect on the opportunities and privileges I’ve been afforded that have helped me along the way.

I defended my PhD in mid March 2015, and within two weeks had driven with Lee from Vermont to Montana, flying back to VT for the ceremony in May. In those 5 years, I moved to Montana, then Oregon, then Maine; I’ve worked for 4 different departments in 3 Universities; I’ve had several different hair styles; I adopted a dog, got married, and bought a house; applied to dozens of jobs almost every year because of the short-term appointments I held; and established a research lab at the University of Maine as an assistant professor. It’s been a pretty busy 5 years, all around. I look forward to the next 5 years, and the opportunity to help the next generation of researchers begin their journey.

Woman with yellow background in a video meeting.

Microbes and Social Equity presentation at IHBE Build Health 2020 virtual meeting

I presented at my first virtual conference; the Institute for Health in the Built Environment Build Health 2020 industry consortium meeting on May 14, 2020.

Comprised of the Biology and the Built Environment Centerthe Energy Studies in Buildings Laboratory, and Baker Lighting Lab, IHBE connects researchers, practitioners, and designers engaged in creating healthier buildings. For the past few years they have hosted a mini-conference in Portland, Oregon in May, but this year a virtual format was a safer choice.

IHBE meeting organizers did a fantastic job at facilitating a remote meeting with a dozen speakers across multiple time zones. This included creating formatted slide decks for speakers to populate, coordinating sections by colors and symbols and providing respective virtual backgrounds for section speakers to use, and use of breakout rooms for smaller discussion groups.

I presented “Framing the discussion of microorganisms as a facet of social equity in human health“, and you can find a recorded version of the presentation here. There are no closed captions, but you can read the audio as annotations here:

The concept of “microbes and social equity” is one I’ve been playing with for a little over a year, and has developed into a colloquium course at the University of Oregon in 2019, an essay in PLoS Biology in 2019, and a consortium of researchers participating in “microbes and social equity part 2”. The Part 2 group has been developing some exciting research events planned for later in 2020, and those details will be forthcoming!

2019 review one of Indoor Air’s top downloaded articles for 2018 – 2019!

A collaborative review article that I was last author on was listed in the top 10% most downloaded papers of 208/2019 in the journal Indoor Air! Even more impressive, this review was published August 20, 2019, and it was still in the top 10% spanning from January 2019 – December 2019!!

This paper stems from my work on the microbiology of the built environment at BioBE, and reviews the interaction between chemistry, microbiology, and health in the built environment. It was co-authored and led by undergraduate students I was mentoring at the time, as well as research associates and PIs from the BioBE lab, and a variety of fabulous collaborators!

From one species to another: A review on the interaction between chemistry and microbiology in relation to cleaning in the built environment

Joined the graduate faculty in Ecology and Environmental Sciences!

I’m pleased to announce that I have been approved for full Graduate Faculty status in the Ecology and Environmental Sciences Program at the University of Maine! EES is an interdisciplinary program that allows for flexibility of scope in research and graduate study. I am now able to:

  • advise PhD, MS, and non-thesis MS students in EES
  •  serve on the graduate committees of EES students
  • design and teach EES-designated grad and undergrad courses
  • advise EES undergrads
  • advise EES honors committees
  • apply for University graduate awards through EES

DNA double helix with dollar signs as a nucleotide.

Extrava-grant-za!

Today a large-scale federal grant proposal was submitted, bringing me to four proposals submitted so far in 2020 (and eight total in the 2019/2020 academic year)! I have one more that is planned for the end of May, and two more that may be submitted this summer depending on the disposition of my pending proposals. Each of these proposals takes weeks to months of planning, writing, and coordination between the research team. The proposal submitted today was 107 pages, and only some of those materials can be re-used between grants, such as descriptions of equipment and research facilities.

A stack of papers facedown on a table.
So. many. supporting documents.

The success rate for obtaining federal funding for your project varies by agency, year, and category of project/principal investigator, nicely tracked here (updated Dec 2019), and currently ranges from 8 – 30%. For example, “pilot” project (small projects to “seed” your long-term research), student-specific, or “new investigator” grants may have a higher rate of success because their applicant pool is restricted by eligibility. Competition is fierce, especially when federal agency budgets are cut or re appropriated.

If projects are not funded, they are returned with reviews from typically 2 – 4 experts in the field who provide comments and recommendations for strengthening the experimental design, or the presentation of the project itself. You might think that proposals are judged on the merit of the science alone, but the ability of the team to manage the project, and the research team, is also being evaluated. Principal investigators (researchers like me, leading the project) need to show that we have good ideas and the organizational skills to implement them, especially if the project spans multiple years or institutions.

Submitting a research proposal is worth celebrating – it represents weeks of effort – but especially during this time when we are all trying to keep our head above water, never mind accelerate or productivity. It’s important to take a few minutes to relax, work can wait, because ‘the grind’ will be there waiting for you when you get back.

Picture of a woman smiling and hanging an arm off of a piece of lab equipment. She is wearing an olive green 500 Women Scientists t-shirt over a black collared long-sleeved shirt.

Spring 2020 Updates

2020 has … gone in a very different direction than the way we probably all thought that it would back in early January. The emergence of the SARS-CoV-2 (COVID-19) pandemic has dramatically altered the way we live our day-to-day lives, and the way we think about ourselves as a global community. To reduce the transmission of the virus, the University of Maine, and many other schools and institutions, made significant alterations to their operating policies over spring 2020. This included sending students home (where possible), moving classes to online instruction only, asking faculty to work from home, and restricting laboratory and field work. This has resulted in some disruption to my plans, so here’s an attenuated post about updates over the last few months.

Teaching

The courses I am teaching this spring lent themselves well to being taught strictly online, with some modifications. Naturally, the presentations class works better in person where the stress of having an audience present promotes in situ training. The students were able to give an elevator speech, a regular short presentation for a peer-level audience, and a peer-level audience presentation with random technical challenges introduced by me into the slides. The remaining portion of the semester was devoted to giving presentations to a public-level audience, which requires a different presentation style and a good deal of thought into how much info is condensed and what you can and can’t expect your audience to already know. It was going to be too logistically challenging to organize public presentations remotely with short notice, so instead I had students create annotation notes for someone else’s slides, described here.

The data analysis class was easier to adapt, but required adjustment nonetheless. Instead of hosting a three-hour video meeting each week, I recorded the remainder of my lectures and made them available well in advance, so that students could watch and listen when they had time and internet access. During the class period, the class met to collaboratively work through data, which was always the goal, but with the challenge of remote work some re-imagination of the assignments was needed to allow students to opt-in to some of the work at times convenient to them.

To simplify the work, instead of having students independently perform similar analyses on different sets of data, I had them perform similar, somewhat independent, analyses on the same dataset, allowing them to all work collaboratively. As a bonus, this unpublished dataset was one that I have been working on collaboratively over the past year, so the students will be able to opt-in to participating in the publication of this work. This is in addition to the two manuscript which are slated to be submitted for review in the next few months, and two more under development. Because that’s still in development, I won’t share more detail now, but stay tuned to those results, and a more in-depth discussion on integrating student data analysis education with research.

Looking ahead, I am making plans to teach my fall classes online as well, including AVS 254: Introduction to Animal Microbiomes, and AVS 401 Senior Paper.

Research

Since starting at UMaine in September 2019, I have been working on establishing my lab. Most of that effort thus far has revolved around rearranging my lab spaces and acquiring specialized lab equipment. This aspect hasn’t been negatively impacted, outside of the new logistical challenges of delivering large pieces of equipment to locked buildings without coming into contact with delivery drivers.

However, acquiring supplies has been impacted, as certain materials are suddenly in extremely high demand, while production of others has dramatically reduced for the time being. Although I hadn’t begun any wet lab research which needed to be halted, I was just about to start culture work and training students on laboratory protocols, which has now been delayed for at least two months. Instead, I am in the process of transitioning student projects from benchtop-based to data analysis-based, at least for those students planning on graduating between now and spring 2021.

I am also focusing on trying to get previously completed projects written up and sent out for review, including a study on bacterial communities around window components in hospital rooms with the BioBE lab, a few collaborations on gut microbes in different animal species with the Yeoman Lab, and two more papers on the effect of climate, farming system, weed competition, and plant health on wheat production and wheat-associated soil bacteria with the Menalled/Seipel Lab. You can read the pre-print (meaning it hasn’t been peer-reviewed yet) on the soil microbes one here.

I do have some concrete exciting news, though, two graduate students will be joining my lab this summer/fall to start work on master’s of science degrees! Johanna Holman will be working on diet and gut microbiome in humans, for a master’s in Nutrition and Food Science, and Sarah Hosler will be working on new methods for investigating gut microbial communities in animals, for a master’s in Animal Science.

Looking forward, I’ll be changing my plan for training students on laboratory work, to facilitate social distancing measures while ensuring that students aren’t alone in the lab. Luckily for me, my labs and soon-to-be-office have windows between them so I can hover from a different room entirely.

Outreach

Social distancing has temporarily impacted my outreach activities, particularly in the short term as we try to adjust. A lecture I had planned for the Maine Organic Milk Producers annual meeting was canceled in April, although I’ll now be able to talk about Microbes and Social Equity at the Institute for Health in the Built Environment Industry Consortium annual meeting in May since it has been moved from in-person to online. Similarly, I am participating in a few discussions for other summer events and whether they might be transitioned to online formats. In the mean time, I’ve been practicing coming up with pithy interactions on Twitter.

A number of scientific conferences which I was planning to attend and/or have research presented at have made the decision to postpone, including the American Society for Microbiology’s Microbe 2020, International Society of Microbial Ecology’s ISME18, the Gordon Research Conference’s Microbiology of the Built Environment (MoBE) 2020 meeting, the American Society for Nutrition 2020 meeting. Other scientific conferences are attempting to switch to online formats, such as the Ecological Society of America (ESA) 2020 meeting, but bringing thousands of participants together in an interactive way is an extremely ambitious adaptation in such a short period of time.

Looking forward, I hope that many organizations will adjust and maintain their commitment to online accessibility of conferences, meetings, talks, and other outreach events, as well as making these resources available after the event. Attend a conference or public presentation is important to building you research program and improving the impact of your work, but financial, physical, logistical, or familial considerations often make it impossible to participate. Maintaining remote-accessibility, and making content available after the event, are important steps in making science more inclusive and allowing a broader audience to participate.

An image of a microbiology and genetics laboratory.

Establishing a research laboratory

As a new assistant professor at the University of Maine, 50% of my appointment is research. To establish my research, I started with curating a space to fulfill the needs of my work — “professional nesting”, if you will. I was allotted two adjacent rooms for my lab work, one as a microbial culturing space, and one for genomics work. I asked for and was granted separate spaces to reduce to likelihood of contamination sourced from my culturing space.

Prior to my arrival at the University of Maine, both lab spaces were set up to perform different research from what I do. This may not seem like it would interfere with my work, but the type of research you do will influence the machinery you need, each of which may have space or utilities requirements, as well as the flow of traffic through the room. To reduce the amount of time you spend moving around the room in search of elusive supplies, it’s best to curate work stations within the room. To that end, the Ishaq lab team spent several days re-arranging the large machinery and the table-top equipment, and then moving the supplies to the cabinets in corresponding locations. This change was most evident in the genomics room, that was previously used for human cell culture and biochemistry, shown below. At this time, I’m still working on updating the microbial culture room, which is larger and contained many more bits and pieces to organize.

  • An image of a microbiology and genetics laboratory.

Most research labs use extremely specialized equipment and machinery. Some of this was made available to me immediately; when research labs are discontinued, ownership of equipment and consumable materials reverts back to the researcher’s home department. I needed to purchase some of the more research-specific equipment, using some of the funds allotted to me for this purpose. Buying equipment can be stressful, because it can be incredibly expensive, and you want to be sure you selected the machine brand and range of capabilities for what you might want to do over the next 5 – 10 years, at least.

Finally, you need to stock your lab with reagents and researchers, but both of these have been temporarily put on hold as of March 2020, as we do our part to reduce the transmission of the Covid-19 virus. Whenever it is safe to do so, I look forward to completing the updates to my spaces and opening them up for collaborative work.

Welcome new lab members!

I’m delighted to officially announce that the Ishaq Lab is welcoming two new graduate students this year, Johanna Holman and Sarah Hosler! Both of them will be working on dynamics of the gut microbiome, and starting a master’s of science this summer/fall. Johanna and Sarah are wonderful additions to our current, enthusiastic team, and the Ishaq Lab can’t wait to see what you achieve!

Johanna Holman

Fall 2020 Masters of Science, Human Nutrition and Food Sciences

A sheep posing for a photo.

Johanna is joining the lab in Fall 2020 to investigate the effects of diet on the gut microbiome, and on host-microbial interactions.

Sarah Hosler

Fall 2020 Masters of Science, Animal Science

Photo of Sarah Hosler in an elevator.

Sarah is from Newton, NJ and has lived in NJ her whole life until she started college at Albright College in PN. Her undergraduate research was on protein classification. Sarah is joining the lab in Fall 2020 to create new methods for studying host-microbial interactions.

“I am excited to see how much I grow through my graduate experience at the University of Maine. I’m also a little bit nervous about the cold, but I’m sure I will get used to it.”

Sarah, Apr 2020
Woman dressed in a costume of a dissected cat, to teach a class on Halloween.

Teaching students to give scientific presentations

This semester at UMaine, I’m teaching a section of AVS633/FSN671 Graduate Seminar, for students in the Animal and Veterinary Science and the Food Science and Nutrition grad programs. Naturally, I decided to spice up the course requirements.

In all the presentations I have given; during classes, teaching, as public lectures, guest seminars, and conference proceedings, I’ve faced a great deal of technical and audience-related challenges. There is a wealth of information on the formatting and content aspects of building a scientific presentation, but in my experience, that’s only half the battle. The other half is in being able to accurately and interestingly relay that information to your audience. Even in professional settings, I have faced disruptive technical failures that caused me to alter my talk or have to adjust my narrative, and I have fielded poorly-crafted or poorly-intended questions from my audience, all while trying to maintain my composure.

I felt that this was what the graduate students needed to learn, and in a safe space where it was OK to simply, well, give a bad presentation. To convey this, I put together an introduction to the class (below) and a series of assignments.

The Elevator Speech

Their very first assignment was to stand up, with notes but no slides, and give a 3 minute speech on a topic of their choice. It had to be non-technical, and designed to provide information in an approachable way such that the person stuck on the elevator with you would actually want to hear more. As academics, especially when you are a student, you often get caught up in repeating jargon or with having to explain yourself in highly detailed language to faculty who are training and testing you. You forget how to present your work to someone who has absolutely no background, and only a few minutes worth of attention span to devote to hearing about your very niche research question. To give an effective elevator speech, the students needed to distill only the critical information for someone to follow their line of thinking, and to not get bogged down by extraneous detail.

Peer Presentations and Awkward Audience Questions

For the second assignment of the course, each student was required to give a presentation on their research, their program of study, or a specific topic they were interested in and the relevant research. Due to the number of students and course time allotted, this presentation only needed to be 10 minutes long, but I’ve found it can be more difficult to present your material concisely. The students presented as if to a peer audience, so they could use a certain amount of jargon or introduce methods with minimal explanation. This style of presentation is common in graduate school, and as expected, the students all did incredibly well.

To add a challenge here, I instead focused on the audience (in this case, the rest of the class). The thing about being an audience member that most people never think about, is that you also need to conduct yourself with a certain level of professionalism. It might not be polite to shout a question or snarky response in the middle of a presentation, your comments might seem complementary but are in fact back-handed, or your question might simply be poorly crafted. I have been asked, or been witness to, a lot of poorly-worded audience questions and responses, and I’m not referring to general public audiences, I’m talking about academics who should know better.

To that end, for each student presentation, I gave an index card to another student in the audience to ask or perform during the talk. Participation was voluntary. Some of these are well-meant questions that are simply commonly asked. Others are silly, and some are rude. I didn’t include anything offensive or abusive, but those examples abound. The list is pretty funny, but please, NEVER DO THESE AS A REAL AUDIENCE MEMBER.

  • Ask the speaker if they will be a medical doctor (or veterinarian) after they finish this [research] degree.
  • State that you have a question. Then pose a statement/comment that is not a question.
  • Be on your phone (texting) or overtly not paying attention to the entire presentation.
  • Ask them to explain a simple concept that they covered in their presentation (but that you missed because you weren’t paying attention).
  • Cough or sneeze comically loud, or drop something during the presentation.
  • Ask the speaker how they chose this topic or how they got into this type of research/work. (This seems benign, but can take away from more specific questions during a peer presentation.)
  • Ask if the speaker is familiar with a field/event/discovery that is somewhat related to their presentation but not actually in their presentation.  Example, speaker presents about infectious disease in cattle and you ask them about “cow farts and global warming”.
  • Comment that the speaker looks really young for someone in their position.  Example: “Wow, I thought you were an undergrad! You look really young. I mean, that’s a compliment.”
  • Get up during the presentation and adjust the lights or shades in the room. You don’t have to make them better, just change them.
  • Ask the speaker a multiple part question. They can be simple questions, but ask them all in one, long, run-on sentence.
  • Begin your question with “As a parent,….” even if you are not a parent and the question has nothing to do with being a parent. 
  • Ask the presenter who analyzed their data for them (even if they have already said they analyzed it themselves).
  • Tell the speaker that their method is not valid (but don’t explain why).
  • Tell the speaker: “This was a pretty good presentation. When you have been in grad school a few more years I think you’ll be a really good speaker.”
  • Tell the speaker that this kind of work has been done before and ask what they have done that is unique.
  • Raise your hand to ask a question, but then sit back, squint your eyes, exhale loudly, pause for a moment, then say, “Never mind”.

The Technical Challenge

On multiple occasions, I have had to give a short (10 min) presentation by memory because the slideshow wouldn’t open or advance. I have had poor lighting, or poor color contrasting from the projector, which made it difficult to read my slides. I have had projection screens which were much smaller than I anticipated such that my text was too small to read on figures, and I’ve more or less given up the hope that I will routinely encounter “presenter mode” when using podiums or other people’s machines. I’ve had a projector that kept shorting out during the talk and creating blank screens for 10 seconds, something which you can hear me talk about in the lecture recording but not see on the recorded slides. I’ve had my available time cut in half, had to cut my presentation short because I included too much detail, realized I had poorly organized the presentation of material or forgotten to define a critical aspect, been unable to play videos or animations, had hand-held slide advancers with low batteries, had automatic slide advance turned on by mistake, and more.

When you face these surprises during a talk, you often don’t have the time, never mind the presence of mind, to resolve the problem. You simply have to make the best of it before your time runs out. It helps to know your material, but it also helps to be able to improvise, which is a skill best developed in practice. You might need to fill air time, or reconstruct your presentation on the fly, or make light of the situation to cut the tension in the room. To help my students prepare, I asked them to send me their peer presentation, as I wanted them to use a presentation they had just given and were familiar with. Then, I introduced mistakes into the presentation without disclosing what those might be, only that they would be there.

To think up enough technical problems I could use, I enlisted the help of scientists on twitter. Click on the Tweet below to find the thread and see the other contributions from @HannahMLachance, @canda007, @Wymelenberg, @vaughan_soil, @murphyc1928, @cskrzy, @maria_turfdr, @mcd_611.

I came up with this non-exhaustive list:

  • Replace a video with a still shot
  • Have 2 students make slides on the same topic, then have them present the other one’s slides (to simulate when a co-author gives you some slides on their contribution and you forget what they mean).
  • Reorder some of the slides
  • Remove a lot of the text on the slide
  • Resize images to be too small for audience to see resolution
  • Introduce blank slides to simulate projector connection issues (like screen flickering on/off occasionally)
  • Ppt won’t open at all or won’t advance beyond title slide
  • Change font on all text to tight cursive
  • No ‘presenter mode’ available
  • Resize slide dimensions and don’t adjust proportions to ensure fit
  • Turn laptop around so can’t see screen as if presenting at a podium
  • Add animations to everything
  • Add notification of email on timer (created a shape with animated pop in and out, as well as notification chime).
  • No photos
  • Slide advancer with poor quality batteries
  • Automatic slide advance

Public Presentations

Public presentations are an overlooked part of academia, but a crucial aspect. If you are at a public university, or you receive state or federal funding, your work is being supported by tax dollars. Many federal grants require an outreach or public education portion to your project, where you make the results available to interested parties (called stakeholders). Science communication is also extremely important in bridging the divide between scientific and public communities.

Public presentations need to present information approach-ably. I don’t mean they need to talk down to people, I mean they need to consider that the audience might not have a frame of reference for what you are talking about. I have a PhD, but it’s meaningless if I attend technical lectures on physics. For the third challenge in class, students can give their presentation again but with the knowledge that they can’t throw 20 slides worth of dense information at their audience, they can’t use technical language without defining it, and that sometimes the best way to explain complicated information is using pictures or analogies.

Update: In light of Corvid-19 concerns, campuses have been closing and switching over to remote instruction. This was rather challenging to do well with a presentations class, as giving a webinar isn’t the same as giving a public presentation. To be more creative, I am having students submit their public presentation slides online. I then assign them to another student, who has to annotate the ‘presenter notes’ with the speech of how they would present these slides. I then return the annotated version to the original presenter so they can see how well their slides spoke for themselves. In this “presentation telephone game”, I hope they will see how easy their slides were translatable to someone else, which is a common problem in slides put online without any notes or audio: so much gets lost when the presenter isn’t providing the information and filling in the additional information that is only briefly noted on the slides.

Learning (to Pretend) to Enjoy Giving Presentation

You can’t always control the technical aspects of your talk, or select your audience, or even be prepared for the weather that day. You won’t always be well-rested, or in good health, on the day of. Fun fact about stress, it can trigger spotting or early menstruation. There’s nothing quite as terrifying as being in the middle of your presentation when you are suddenly aware that you have a limited amount of time to get off stage and hope that there are feminine products available for free in the nearest restroom, because your women’s dress pants don’t have pockets for you to carry quarters for the dispensary machines.

You won’t always have time to prepare. Once, I had 5 minutes of notification that I would have to stand up in front of 50 – 75 other college students and Jane Goodall and present a recap on a service-learning course, at a time when I dreaded any and all public speaking. But you can’t really decline the offer to talk in front of Jane Goodall when she had taken the time and effort to be in the room to listen to you all. So you just have to stand up and start talking before you convince yourself you can’t do it.

You can have faith in yourself, know that you will try your best, and remind yourself that it will be good enough. I’ve been an audience member at perfect presentations, and I remember that it went really well and nothing at all about the content. The talks that I remember most are the ones where the speaker connected with me. They were funny, they were humanizing, and they took technical problems and awkward interactions in stride.

The best way to become a better speaker, I think, is to be open to the idea that you are going to mess up. A lot. But each time, you will learn from that experience, you will ask for feedback, and you get back out there. As academics, we have to present information on nearly a daily basis. It is, in fact, a significant part of the job. So instead of dreading it, we should at least pretend to enjoy it until, one day, we find that we do.

Image of plastic wrapped over soil to inhibit weed growth.

NE IPM funded collaborative proposal!

I’m pleased to announce that a small grant proposal I am part of was just funded by the Northeastern Integrated Pest Management (IPM) Center! The proposal, “A Working Group on Tarping and Soil Solarization”, brings together researchers and food production professionals from across New England to identify the current use of tarping and soil solarization to prevent weed growth without the use of chemcials, as well as identify barriers to adoption of this practice, and develop research proposals to fill any knowledge gaps related to the use of these methods and their effect on crop production, weed suppression, soil microbiota, and the local ecosystem.

Led by Dr. Sonja Birthisel (UM), the working group team is comprised of Dr. Alicyn Smart (UM), myself, Master Nathalie Lounsbury (UNH), and Eva Kinnebrew (UVM). We will be joined by over a dozen other researchers across New England who perform agricultural research, along with dozens of ‘stakeholders’: producers and other food production professionals who have an interest in the group findings and would make use of any knowledge we generate.

Featured Image Credit: Soil Solarization, Wikimedia