My greenhouse trial on the legacy effects of farming systems and climate change has concluded! Over this past fall and winter, I maintained a total of 648 pots across three replicate trials (216 trials per). In the past few weeks, we harvested the plants and took various measurements: all-day affairs that required the help of several dedicated undergraduate researchers.
In case you were wondering why research can be so time and labor intensive, over the course of the trials we hand-washed 648 pot tags twice, 648 plant pots twice, planted 7,776 wheat seeds across two conditioning phases, 1,944 wheat seeds and 1,944 pea seeds for the response phase. We counted seedling emergence for those seeds every day for a week after each of the three planting dates in each of the three trials (9 plantings all together). Of those 11,664 plants, we hand-plucked 7,776 seedlings and grew the other 3,888 until harvesting which required watering nearly every day for over four months. At harvest, we counted wheat tillers or pea flowers, as well as weighed the biomass on those 3,888, and measured the height on 1,296 of them. And this is only a side study to the larger field trial I am helping conduct! All told, we have a massive amount of data to process, but we hope to have a manuscript ready by mid-summer – stay tuned!
As the 2016 growing season comes to a close in Montana, here in the lab we aren’t preparing to overwinter just yet. In the last few weeks, I have been setting up my first greenhouse trial to expand upon the work we were doing in the field. My ongoing project is to look at changes in microbial diversity in response to climate change. The greenhouse trial will expand on that by looking at the potential legacy effects of soil diversity following climate change, as well as other agricultural factors.
First, though, we had to prep all of our materials, and since we are looking at microbial diversity, we wanted to minimize the potential for microbial influences. This meant that the entire greenhouse bay needed to be cleaned and decontaminated. To mitigate the environmental impact of our research, we washed and reused nearly 700 plant pots and tags in order to reduce the amount of plastic that will end up in the Bozeman landfill.
Each pot needed to be scrubbed with disinfectant soap and then soaked in bleach.
Lines of pots drying on the rack.
I scrubbed 700 labels clean in order to reuse them.
We also needed to autoclave all our soil before we could use it, to make sure we are starting with only the microorganisms we are intentionally putting in. These came directly from my plots in the field study, and are being used as an inoculum, or probiotic, into soil as we grow a new crop of wheat.
This is trial one of three, each of which has three phases, so by the end of 2016 I’ll have cleaned and put soil into 648 pots with 648 tags; planted, harvested, dried and weighed 11,664 plants; and sampled, extracted DNA from, sequenced, and analyzed 330 soil and environmental samples!
Each pot gets six tiny winter wheat seeds planted.
Trial 1: 216 pots ready to grow!
Stay tuned for more updates and results (eventually) from this and my field study!